Study on Fingerprint and Determination of Saikosaponin b2 of Radix Bupleurioral Liquids by HPLC

Abstract:

Objective: To establish an HPLC method for a standard fingerprint and determination of saikosaponin b₂ for quality control of Radix Bupleurioral liquids. Method: About 10 samples from different batches were analyzed by HPLC. By the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (Version 2004A), mean chromatogram was generated as the representative standard fingerprint and the similarity of each chromatogram against the standard chromatogram was also calculated. Result: Eleven main marker peaks were selected in the standard fingerprint and 1 peak of them were identified. The similarity of all samples was over 0.985.The linear range of saikosaponin b₂ was 0.039 7-0.793 3 μg (r=0.999 1). The average recovery was 98.27% with RSD of 1.79% (n=6). Conclusion: The developed method was reliable, and can be used for the quality control of Radix Bupleurioral liquids.