Abstract:Objective: To study the effects of water extracts from Curcuma kwangsiensis on liver detoxication enzymes in cytosol and microsomes in rats. Method: Rats were treated with 0.81, 2.43, 7.29 g·kg-1 once daily for 21 days, serum were collected for determination of alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea nitrogen (BUN), creatinine (Cr), and liver was removed, then cytosols and microsomes isolated from liver were prepared by differential centrifugation according to the standard procedure. Antioxidant enzmes catalase (CAT), superoxide dismutase (SOD), glutathione peroxidose (GSH-Px), glutathione reductase (Gr) in cytosols and glutathione-S-transferase (GST), uridinediphosphoglucuronate glucuronosyl transferase (UGT) in microsomes were determined by UV-V is spectrophotometer. Result: C. kwangsiensis water extract had no effect on weight, liversomatic indexes, ALT and AST activities under different treatment compared with control group (P>0.05), but significantly decreased the level of Cr (P<0.01) in all groups, middle and high doses could also decreased BUN level (P<0.01). Compared with the control group,C. kwangsiensis had no effect on CAT and GR in cytosols. The groups of 2.43 g·kg-1 (P<0.01) and 7.29 g·kg-1 (P<0.05) significantly increased the activities of SOD. Furthermore, all of the C. kwangsiensis groups significantly increased the activities of GSH-Px (P<0.01). Compared with the control group, all of C. kwangsiensis groups had no effect on the activities of UGT. All groups of C. kwangsiensis, significantly induced the activities of GST (P<0.05) in microsomes. Conclusion: C. kwangsiensis have no toxicity on liver and kidney in rats, on the contrary, C. kwangsiensis may have a protective effect on kidney. C. kwangsiensis can induce the detoxication enzyames in cytosols and microsomes of rat liver, it have antioxidant function and increase the detoxication of liver.
