Effect of Kuijieling Decoction Containing Serum on ITF mRNA and MUC2 Protein Expression in Caco-2 Cell Injury Model

Abstract:

Objective: To observe the effect of Kuijieling decoction containing serum on intestinal trefoil factor(ITF) mRNA and mucin 2(MUC2) protein expression in Caco-2 injury model. Method: Three trinitrobenzene sulfonic acid (TNBS) enema was used for replication of ulcerative colitis (UC) rat model. Cell injury model was established by serum of UC model rats, ITF mRNA and MUC2 protein expression were detected by real time fluorescent quantitative-PCR (RT-PCR) and immunohistochemistry. Result: ITF mRNA (1.20±0.16) and MUC2 protein expression levels(11.22±2.37) in model serum group were higher than that in normal group (1.00±0.11,8.62±3.16) (P<0.05,P<0.01); Compared with the model serum group, ITF mRNA(1.76±0.37) and MUC2 protein expression levels (13.94±2.59) in Kuijieling decoction containing serum group increased significantly (P<0.05, P<0.05). Conclusion: Kuijieling decoction containing serum could promote ITF mRNA and MUC2 protein expression in Caco-2 cell injury model.