Effect of Total Organic Acid of Thladiantha dubia on Active Capacity of Isolated Rat Uterine Smooth Muscle in vitro

Abstract:

Objective: To explore the effects of the total organic acid of Thladiantha dubia (FTDBP) on activity of isolated rat uterus smooth muscle in vitro. Method: The effect of the FTDBP on activity of the isolated rat uterus smooth muscle of non-pregnant rats eight weeks old was recorded by Maclab/4e four channels physiological recorder. Four antagonists, propranolol hydrochloride tablets (3.4×10^-7mol·L^-1),diphenhydramine hydrochloride tablets (2×10^-6 mol·L^-1),ranitidine hydrochloride tablets (2×10^-5 mol·L^-1) and atropine sulfate tablets (2×10^-5 mol·L^-1) were used to study their mechanism respectively. Result: Compare with model group, the FTDBP markedly inhibited the tension, frequency and activity of uterus of rat in vitro.And the IC₅₀ of FTDBP was 0.100 24 g·L^-1. Conclusion: The effect of FTDBP on activity of uterine smooth muscle in rats is mainly associated with M receptor but not H₁ receptor, H₂ receptor or β receptor.