Study on Inhibition of Gardeniae Fructus Extracts on Alpha-glucosidase

Abstract:

Objective: To study the inhibitory effects of Gardeniae Fructus extracts on alpha-glucosidase activity. Method: The inhibitory activities of each extracted parts of Gardenia jasminoides against alpha-glucosidase were tested by the colorimetry of PNPG, the reaction system of which included 67 mmol·L^-1 potassium phosphate buffer 870 μL, 1 g·L^-1 glutathione solution 25 μL, 2.74 U·mL^-1 α-glucosidase solution 35 μL, 15 mmol·L^-1 PNPG solution 70 μL, 0.1 mol·L^-1 Na₂CO₃ solution 4 mL. Result: At concentrations of 50, 100 g·L^-1, Gardeniae Fructus total extract, iridoids and gardenia yellow reduced significantly the activities of alpha-glucosidase with inhibitory rates of 61.47%,83.20%; 34.27%,55.73%; 78.13%, 96.93%. Conclusion: Gardeniae Fructus extracts have the inhibitory effects on alpha-glucosidase, and the active position is probably Iridoids and Gardenia yellow.