Influence of Jianpi Yiqi Huoxue Decoction on Vasomotor Factors Expression in Vascular Endothelium of Spontaneously Hypertensive Rats

Abstract:

Objective: To study the influence of Jianpi Yiqi Huoxue decoction on systolic pressure and vascular endothelium vasomotor factors of spontaneously hypertensive rats(SHR). Method: Sixty 24-week-old SHRs were randomly divided into six groups: SHR group (distilled water), perindopril group (perindopril 0.4 mg·kg^-1·d^-1), perindopril plus herb group (perindopril 0.4 mg·kg^-1·d^-1+20 g·kg^-1·d^-1 of herb), high, medium and low dose group (40,20,10 g·kg^-1·d^-1).of Jianpi Yiqi Huoxue decoction and Wistar kyoto rats(WKY) group. The changes of systolic pressure were measured by BP-98A intelligent sphygmomanometer. After six weeks of treatment, the concentration of nitric oxide(NO), prostacycline(PGI₂),endothelium derived hyperpolarizing factor(EDHF), angiotensin Ⅱ(AngⅡ), and endothelin(ET-1) in plasma of the rats were determined. Result: The systolic pressure of the perindopril group and the perindopril plus herb group was prominently decreased, compared with that of the SHR group (P<0.01). The concentration of NO, PGI₂,and EDHF of the SHR group was lower than those of the WKY group, the perindopril group, the perindopril plus herb group, and the two herb dose groups(P<0.01). The concentration of ET-1, AngⅡ in the SHR was higher than those in the WKY group(P<0.05), and was significantly higher than those in the perindopril group, the perindopril plus herb group, and the three herb dose groups. Conclusion: The Jianpi Yiqi Huoxue decoction may improve functions of blood vessels, and reduce systolic pressure by regulating the balance between the secretion and release of vasomotor factors in vascular endothelium so as to modify the vascular endothelial function obstacles caused by hypertension.