Antioxidant Activity of Different Extracts from Rourea microphylla in Guangxi

Abstract:

Objective: To study the antioxidant activity of different extracts from Rourea microphylla in Guangxi. Method: EE (95%ethanol), AE(acetone)and EAE (ethyl acetate) extracts were obtained by cold-extraction. The antioxidant activities were studied using DPPH(1,1-diphenyl-2-picrylhydrazyl radical 2,2-Dipheny l-1-(2,4,6-trinitrophenyl) hydrazyl)assay, 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS⁺) assay and reducing power assay by UV-VIS (Ultraviolet and visible spectrophotometry) spectrophotometry. Result: The EE of R. microphylla exhibited the most outstanding antioxidant activities. The IC₅₀ values of EE in DPPH assay and ABTS⁺ assay were 0.66,0.60 g·L^-1, respectively. The reducing power of all extracts was in the sequence of EE>EAE>AE, which was in accordance with the total flavonoid(TF)content. Conclusion: All the extracts especially EE extract of R. microphylla could be employed as natural antioxidants and health care products.