Effects of Ethanol Extraction from Cassiae Semen on Leptin and NPY in Hyperlipidemia Rats

Abstract:

Objective: To observe effects of ethanol extraction from Cassiae Semen on leptin and neuropeptide Y(NPY) in hyperlipidemia rats. Method: The rat hyperlipidemia model was established by feeding high calorie-diet. After modeling, the selected rats were randomly divided into six groups: normal control group, model group, positive control group, low dose group, medium dose group and high dose group. Positive control group was administrated Xuezhikang capsule at dose of 0.15 g·kg^-1. Low dose group, medium dose group and high dose group were administrated at different concentrations of ethanol extraction of Cassiae Semen, which was respectively 4,8,16 g·kg^-1. All groups were applied by intragastric administration for 4 weeks. Except normal control group, the remains were fed with high calorie-diet. After the last administration, the serum content of total cholesterol (TC), triglyceride(TG), low-density lipoprotein-cholesterol(LDL-C), high-density lipoprotein-cholesterol(HDL-C) and glucose(Glu) were detected by automated chemistry analyzer. And the content of leptin and NPY was determined by ELISA. Result: The content of TC, TG, LDL-C and Glu was decreased, and the content of HDL-C was increased in medium dose group and high dose group of ethanol extraction of Cassiae Semen, compared with model group(P<0.01). At the same time, the content of leptin and NPY was decreased in medium dose group and high dose group(P<0.01). Conclusion: Ethanol extraction of Cassiae Semen can regulate the metabolic process of blood lipids and blood glucose levels of hyperlipidemia model rats, which might be contributed to its effect on leptin-hypothalamic neuropeptide Y chain.