Effect of Total Flavonoids of Clerodendranthus spicatus on Chronic Abacterial Prostatitis in Rats

Abstract:

Objective: To study the therapeutic action and mechanism of total flavonoids of Clerodendranthus spicatus (TFC) on rats models of chronic abacterial prostatitis. Method: Sixty healthy adult male SD rats were randomly divided into six groups, 10 rats in each group: blank control group, model group, positive control group (250 mg·kg^-1·d^-1), TFC low-dose, middle-dose and high-dose group (100, 200, 400 mg·kg^-1), respectively. Except for normal group, the rats models of Chronic abacterial prostatitis were builded by subcutaneous injection of oestradiol monobenzoate at the dose of 0.25 mg·kg^-1 after castration, and then treated with TFC at 100, 200, 400 mg·kg^-1 by oral administration whileblank and model group were given normal saline, once a day for 30 days. White blood cell(WBC) count, lecithin corpuscle density and prostate index in prostatic fluid were examined, and the expressions of tumor necrosis factor(TNF-α) and interleukin-8(IL-8) in the serum and prostate tissues of the rats were detected by ELISA. Result: Compared with model group, WBC count and prostate index were reduced and lecithin corpuscle density were increased in prostatic fluid by TFC, which showed significant statistical differences(P<0.01 or P<0.05), and the levels of TNF-α and IL-8 in the serum and prostate tissues of the rats were reduced. Conclusion: TFC has therapeutic effects on chronic abacterial prostatitis inrats models, and the mechanism may be related to decreasing the levels of TNF-α and IL-8 in the serum and prostate tissues.