Abstract:Objective: To observe the chronic toxicity damage of the water extraction components from Evodia Fructus in rats. Method: The water extraction components with different doses from Evodia Fructus were separately orally given to rats, and the high, middle and low lose-group were separately 8.0,4.0,2.0 g·kg-1 calculated by crude herbs, the general conditions were observed and the related indexes were detected, such as the routine control of blood, hepatic function, renal function, the metabolism condition of lipids and the glycometabolism indexes; the rats were dissected and precisionly weigh the weight of heart, liver, spleen, lung, renal,and calculated the ratio of organ to body; the histopathologic examination of hepatic tissue were examined. The remaining rats were not administrated, and recovered for 20 days, the recovery condition of rats were observed,and the above indexes were detected. Result: The water extraction components from Evodia Fructus with different doses could induce the body weight decreasing in rats, the level of alanine aminotrans ferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP) and total protein(TPC) serum obviously increased, albumin(ALB) and the ratio of ALB to GLB in serum obviously decreased, the liver weight and the ratio of liver to body increased, and the hepatic tissue damage was obvious in the histopathologic examination of hepatic tissue;the above-mentioned changes gradually aggravated with dose increasing, and it was the obvious discrepancy compared with control group. The influence on the content of total bilirubin(TBI),blood urea nitrogen(BUN), glucose(GLU),total cholesterol(CHO) and creatinine(Cr) was not obvious, and the hepatic toxicity degree had certain dose indepdence relatonship. The ALT level of high dose group in the above indexes of recovered rats had significant difference compared with control group, and the other indexes had no significant difference. Conclusion: The long term administration of water extraction components from Evodia Fructus at the certain dose in rats could induce obvious cumulative toxicity, and the mainly toxical injury sites were in liver, but after recovery period observation, the toxicity was basically recovered.
