Preliminary Exploration of Industrialization for Purification of Hydroxysafflor Yellow A from Carthamus tinctorius by Macroporous Resin

Abstract:

Objective: To optimize industrialization amplification process for purification of hydroxysafflor yellow A from Carthamus tinctorius by macroporous resin. Method: The content of hydroxysafflor yellow A was determined by HPLC,single factor tests were adopted to investigated effects of pH of sample solution,the concentration and amount of eluant,quality ratio of raw materials to resin on purification technology,then verified optimized technology through pilot and industrialization production. Result: Optimum purification conditions were as follows:ratio of crude drug to D101 resin 1: 6,pH of sample solution 4.0,eluted impurity with 4 BV water at flow rate of 3 BV·h^-1,then eluted with 6 BV 5% ethanol at 3 3 BV·h^-1,collected eluent. Conclusion: This technology was simple and feasible with good separation,it could be suitable for industrialized production of hydroxysafflor yellow A.