Optimization of Alcohol Precipitation Technology of Polysaccharides from Sophora alopecuroides by Response Surface Methodology

Abstract:

Objective: To optimizate alcohol precipitation technology of polysaccharides from Sophora alopecuroides. Method: Based on single-factor test,with yield of polysaccharides as index,precipitation time,the concentration and adding multiples of ethanol was optimized according to mathematic model built by central composite design in Design Expert software on principle of response surface methodology.The content of polysaccharides from S. alopecuroides was determined by phenol-concentrated sulfuric acid method. Result: Optimum alcohol precipitation technology was as following:precipitation time of 26 h,the concentration of ethanol 97%,adding multiples of ethanol 4.3 times.Under these conditions,yield polysaccharides from S. alopecuroides was 6.98% while the predicted value was 7.03%,their relative error was 0.71%. Conclusion: Response surface methodology was effective to optimize alcohol precipitation technology of polysaccharides from S. alopecuroides.