In vitro Transdermal Test of Yinhu Ganmao Spongia

Abstract:

Objective: To investigate in vitro transdermal absorption properties of Yinhu Ganmao spongia. Method: The content of chlorogenic acid was determined by HPLC and GC determination of linalool content,release was detected by Franz diffusion cell method with 10% ethanol as receiving liquid,transdermal receiving liquid at different time points was collected,and calculated cumulative permeation amount and ratio. Result: Regression equations of cumulative permeation amount of chlorogenic acid and linalool were Q=7.143 8t+8.229 4(r=0.996 1),Q=125.375t-143.705(r=0.989 8),respectively.J of them were 7.144,125.375 μg·cm^-2·h^-1,respectively. Conclusion: Chlorogenic acid in Yinhu Ganmao spongia continuous permeated uniformly within 24 h,but linalool almost permeated compeletly in 12 h;This method was simple and rapid,it was a ideal method to comprehensive evaluate in vitro transdermal performances of Yinhu Ganmao spongia.