Preparation and Quality Conrol of Andrographolide Liposomes

Abstract:

Objective: To prepare andrographolide liposomes and establish its quality control method. Method: Andrographolide liposomes was prepared by film dispersion-ultrasound method,with encapsulation efficiency as index,orthogonal test was adopted to investigate effects of ratio of soybean lecithin-cholesterol,ratio of soybean lecithin-andrographolide,the amount of vitamin E and ultrasonic time on preparation technology of andrographolide liposomes.Particle size and morphology of these prepared liposomes was assessed.The content of andrographolide was determined by HPLC. Result: Optimum preparation technology was as following:ratio of soybean lecithin-cholesterin 6: 1,ratio of soybean lecithin-andrographolide 4: 1,the amount of vitamin E 20 mg,ultrasonic time 5 min.Morphology of prepared liposome showed sphere structure with uniform diameter.The average particle size was 625.8 nm and encapsulation efficiency was 81.35%,rate of leakage was 1.54%.The linear range of andrographolide was 8.16-48.96 mg·L^-1(r=0.999 8). Conclusion: Prepared andrographolide liposomes had high entrapment efficiency and low leakage rate,this established method for quality control was reliable,which lay foundation for preparing andrographolide liposomes aerosol.