Optimization of Purification Technology for Total Flavonoids and Cyanogenetic Glycosides in Leaves of Sorbus tianschanica by Macroporous Resin

Abstract:

Objective: To optimize purification technology of total flavonaoids and cyanogentic glycosides in leaves of Sorbus tianschanica by macroporous resin. Method: With absorption capacity,absorption rate and desorption rate as indexes,5 different macroporous resins were screened by static adsorption test,effects of the concentration of sample solution,ratio of diameter to height,adsorption velocity,the concentration of ethanol,elution velocity and other process parameters on purification technology of total flavonoids and cyanogenetic glycosides in leaves of S. tianschanica were investigated by single factor tests. Result: HPD-100 resin was adopted,its optimum purification technology was as following:absorption capacities of crude drugs to resin 2.2 g·g^-1,the concentration of sample solution 0.1 g·mL^-1 with ratio of diameter to height at 1: 6,adsorption velocity at 2 BV·h^-1,standing for 30 min,eluted with 4 BV 50% ethanol,elution velocity 2 BV·h^-1,collected eluent. Conclusion: HPD-100 resin could be used successfully to purify total flavonaoids and cyanogentic glycosides in leaves of S. tianschanica with the mass fraction of effective part in ethanol eluate was more than 50%.