Effects of Different Pretreatment Methods on Vacuum Reflux Extraction Technology of Epimedium brevicornum

Abstract:

Objective: To investigate effects of different pretreatment methods on vacuum reflux extraction efficiency of Epimedium brevicornum. Method: Taking yield of icariin as index,vacuum reflux extraction technology of E. brevicornum was optimized by orthogonal test with vacuum degree,extraction time,temperature and times as factors,single factor tests were adopted to investigate effects of soaking time and crushing degree on vacuum extraction efficiency of E. brevicornum. Result: Optimum vacuum extraction technology was as following:extracted 3 times with 10 times the amount of 60% ethanol,20 min each time,vacuum degree 0.07 MPa.Yield of icariin increased by 9.5% after E. brevicornum crushed into fine powder,it was 7.9% after crushed into coarse powder,and extract yield increased by 2.6%,1.7%,respectively.After soaked different time,yield of icariin increased by 6.4% and dry extract yield increased by 6.9% at most,soaked 20 min had optimal extraction efficiency,but coarse powder and fine powder had no significant differences. Conclusion: Yield of active ingredients and dry extract rate of E. brevicornum would increase under different pretreatment methods.