Determination of Eight Nucleosides in Rhizoma Arisaema, Rhizoma Pinellie and Rhizoma Typhonii

Abstract:

Objective: To establish an HPLC method for simultaneous determination of eight nucleosides in Rhizoma Arisaema, Rhizoma Pinellie and Rhizoma Typhonii. Method: The chromatographic separation was achieved on a Phenomenex Luna PFP (2) (4.6 mm×250 mm, 5 μm) column with methanol-water solution as mobile phase (gradient elution). The flow rate was 0.8 mL·min^-1, the column temperature was maintained at 35℃ and the detective wavelength was set at 260 nm. Result: The linear ranges of uracil, hypoxanthine, xanthine, uridine, inosine, guanosine, thymidine and adenosine were 0.004 34-0.434 μg (r=0.999 9), 0.002 34-0.234 μg (r=0.999 9), 0.004 07-0.407 μg (r=0.999 7), 0.010 90-1.090 μg (r=0.999 9), 0.002 01-0.201 μg (r=0.999 9), 0.007 32-0.732 μg (r=0.999 9), 0.001 22-0.122 μg (r=0.999 7), 0.002 07-0.207 μg (r=0.999 9) respectively. The average recoveries of the eight nucleosides were 104.2%, 98.7%, 103.7%, 99.4%, 101.8%, 102.9%, 98.3%, 102.3% and RSDs were 2.1%, 2.4%, 3.0%, 1.4%, 2.0%, 1.9%,2.8%, 2.7% respectively. Conclusion: The method is simple, accurate and rapid. It can be used for determination of nucleosides in Rhizoma Arisaema, Rhizoma Pinellie and Rhizoma Typhonii.