HPLC Fingerprint of Shixiao San

Abstract:

Objective: To establish the fingerprint of Shixiao San, enhance the quality control of the method. Method: The RP-HPLC was performed on an C₁₈ chromatography column, with acetonitrile-0.1% of TFA as mobile phase, the flow rate was 1 mL·min^-1; the wavelength was set at 360 nm. The temperature of column was maintained at 30℃. Result: The common modes for the HPLC fingerprints were set up by ensuring 15 common peaks in the chromatograms. The similarity degrees in the ten batches were in the range of 0.962 to 0.996. Conclusion: The method is sensitive, accurate and simple, thereby it can be used specifically for the quality control of Shixiao San.