Study on Qualitation and Quantitation of Coptis chinensis in the Qingshen Granule

Abstract:

Objective: To study quality standard of Coptis chinensis in the Qingshen granule. Method: Thin-layer chromatography was used to identidy C. chinensis.The content of berberine hydroc hloride was determined by HPLC.The HPLC separation was performed on kromasil-C₁₈ column (4.6 mm×250 mm,5 μm) with acetonitrile-0.033 mol·L^-1KH₂PO₄(25: 75) as mobile phase; the detection wavelength was set at 345 nm; the flow rate was 1.0 mL·min^-1, and the column temperature was set at 30℃. Result: The spot in the TLC was clear,with no interference from negative control. In the HPLC experiments of precision, reproducibility and stability, the samples could be separated entirely. The linear range of berberinehydroc hloride was 0.028-0.892 μg(r=0.999 9), the average recovery was 100.16% with RSD of 1.87%(n=6). Conclusion: The methods are accurate and quick in qualitative identification and quantitative assay of this preparation,which can be used for the quality control of Qingshen granule.