HPLC Determination of Hyperin in Compound Hypericum Perforatum Dropping Pills

Abstract:

Objective: To establish the method to determine the concentration of hyperin in Compound Hypericum Perforatum Dropping Pills. Method: HPLC was used for the determination of hyperin in Compound Hypericum Perforatum. A COSMOSIL packed column 5 C₁₈-MS-Ⅱ (4.6 mm×250 mm, 5 μm) was used. The mobile phase was acetonitrile-water (0.5% glacial acetic acid and 2% tetrahydrofuran) (13:87). The detection wavelength was set at 360 nm. Result: The linear range for hyperin was 10.8-54.0 mg·L^-1 (r=0.999 1). The average recovery was 98.31% (RSD 1.23%). Conclusion: The method can control the quality of Compound Hypericum Perforatum Dropping Pills effectively. It was sensitive, accurate and feasible.