Simultaneous Determination of Notoginsenoside R1, Ginsenoside Rg1 and Ginsenoside Rb1 in Chinese Formula KLW by UPLC-MS-MS

Abstract:

Objective: To develop an ultra performance liquid chromatography tandem mass (UPLC-MS-MS) method for determination of notoginsenoside R₁, gensenoside Rg₁, and gensenoside Rb₁ in Chinese formula KLW. Method: The Chinese formula KLW is extracted by ultrasonic and centrifugal, then filtered before test. The ultra performance liquid chromatographic separation was performed on a Waters ACQUITY BEH C₁₈ column (2.1 mm×50 mm, 1.7 μm) by gradient elution with methanol-water as the mobile phase at a flow rate of 0.4 mL·min^-1. The analysis of 3 compounds was performed under ESI multiple reaction monitoring (MRM) mode, and the quantification was conducted by external calibration method. The monitoring ions for notoginsenoside R₁ were m/z 1 007.2/423.3 m/z; gensenoside Rg₁ were 875.2/423.5 m/z; gensenoside Rb₁ were 1 183.3/487.3 m/z. Result: The method showed a good linearity in the range of 0.05-2.0 mg·L^-1, with the correlation coefficient above 0.999.The average recoveries (n=6) of notoginsenoside R₁, ginsenoside Rg₁ and ginsenoside Rb₁ were 97.7%, 96.3%, 97.1% respectively. Conclusion: This method is simple, rapid, accurate and sensitive, can also provide a reliable method for clinical research and can be used for the quality control of Chinese formula KLW.