Simultaneous Determination of Retusine and Pogostone in Pogostemon cablin by HPLC

Abstract:

Objective: To establish a high performance liquid chromatography (HPLC) method for simultaneous determination of retusine and pogostone in Pogostemon cablin, and analyze the quality of 11 batches of retusine and pogostone in P. cablin. Method: The RP-HPLC system consisted of a Phenomenex Luna C₁₈ column (4.6 mm×250 mm, 5 μm) and the mobile phase of acetonitrile-formic acid by isocratic elution. The flow rate was set at 0.8 mL·min^-1 and detected at 310 nm; the column temperature was kept at 30℃. Result: The linear regression for retusine and pogostone was obtained in the range of 0.020-0.36 μg (r=1.000 0) and 0.025-0.40 μg (r=1.000 0) respectively; the average recovery was 98.57% (RSD 2.06%) and 100.04% (RSD 2.66%) respectively. The content of retusine and pogostone in 11 batches of P. cablin were in the range of 1.13-8.72 mg·g^-1 and 1.19-4.38 mg·g^-1 respectively. Conclusion: The method is quick, simple and reproducible for the determination of retusine and pogostone in P. cablin simultaneously.