Determination of Artemitin and Chrysosptertin B in Laggera pterodonta

Abstract:

Objective: To establish an effective HPLC method for determining the content of artemitin and chrysosptertin B in Laggera pterodonta. Method: By HPLC, a Diamonsil C₁₈ column (4.6 mm×250 mm, 5 μm) was adopted with methanol-water (60: 40) as the mobile phase for elution at the flow rate of 1.0 mL·min^-1. The detection wavelength was set at 257 nm and the temperature was kept at 32.2℃. Result: Good linearity was obtained in the range of 0.448-2.24 and 0.352-1.76 μg for artemitin and chrysosptertin B respectively. The method recoveries of artemitin and chrysosptertin B were 103.48% and 103.42%, RSDs were 0.87% and 0.91% respectively. Conclusion: The method is simple, accurate and suitable for the determination of artemitin and chrysosptertin B, which can be used for quality control of Laggera pterodonta.