Determination of Naringin and Naringenin in Rat Liver Microsomes by UPLC

Abstract:

Objective: To establish a method for determining the content of naringin and naringenin in rat liver microsomes by UPLC,and preliminary investigation in vitro metabolism of two ingredients at different times points. Method: Determination was performed on UPLC chromatographic system,ACQUITY BEH C₁₈ column (3.0 mm×100 mm,1.7 μm) with a mobile phase of methanol-0.1% acetic acid and gradient elution at a flow rate of 0.5 mL·min^-1,detection wavelength was 283,289 nm,column temperature was 30℃,with quercetin as an internal standard,injection volume was 3 μm. Result: Naringin,naringenin and internal standard quercetin had good separation degree and no endogenous interference.The linear range of naringin and naringenin were 3.814-38.143(r=0.999 2),3.586-35.857 mg·L^-1(r=0.999 6),respectively.Method recoveries of naringin ranged from 95.43% to 97.95% and absolute recoveries ranged from 97.33% to 98.18%.Method recoveries of naringenin ranged from 95.27% to 99.31% and absolute recoveries ranged from 97.71% to 99.73%.I phase metabolism of naringenin in in vitro liver microsomes was more significant than naringin. Conclusion: This method was rapid,sensitive and accurate,it could be used for determination of naringin and naringenin in rat liver microsomes.