Preventive Effect of Haobie Yangyin Ruajian Prescription on Hepatic Fibrosis Induced by CCl4

Abstract:

Objective: To study the preventive effects of Haobie Yangyin Ruajian prescription (HBYYRJ) on rat hepatic fibrosis induced in rats by CCl₄ and explore the effective mechanisms. Method: Rats were randomly divided into 7 groups:normal control group, model group, high,middle and low dose of HBYYRJ groups(8.20,2.59,0.82 g·kg^-1), compound Biejia Ruangan table group(0.54 g·kg^-1), colchicin group(1×10^-4 g·kg^-1). First CCl₄ 5 mL·kg^-1 was given by sc, 40% CCl₄-peanut oil 3 mL·kg^-1 was given by sc every three days from the second time to the twelfth time; 30% alcohol (1 mL) was ig given to model group and therapeutic groups every other day, drugs were given to the rats in therapeutic groups for six consecutive weeks, distilled water was given to the rats of normal and model groups. Histological observation of the liver tissue and collagen content were carried out. Blood serum was used for measuring the contents of alanine transaminase(ALT), aspartate aminotransferase(AST),hyaluronic acid(HA),laminin(LN), type III procollagen(PCIII),type IV collagen(IVC), albumin(ALB). The content of malondialdehyde (MDA) was detected by ultraviolet visible spectrophotometry. The content of hydroxyproline,collagen and albumin were detected in liver tissue. Result: The degeneration and necrosis,inflammatory cell infiltration,false lobular formation,and a large number of collagen fiber depositions were observed in liver tissue of the model group.The level of chronic hepatic damage was improved greatly and none of the false lobular was formed in the high dose of HBYYRJ. The contents of ALT,AST,HA and PCIII of the model group were significantly higher than that in normal group (P<0.01,P<0.05). The content of ALT,AST,HA and PCIII of HBYYRJ groups was significantly lower than that in model group (P<0.05). The content of MDA and collagen in liver tissue of HBYYRJ groups was lower than that in model group (P<0.01). The content of ALB of the high dose of HBYYRJ was significantly higher than that in model group (P<0.01). Conclusion: HBYYRJ has preventive effects on hepatic fibrosis in rats induced by CCl₄.The mechanism might be associated with anti-lipid peroxidation, antiinflammation and restraining the compose of collagen protein.