Effect of Beta-asarone on Adriamycin-induced Cardiomyocyte Injury in Suckling Mouse

Abstract:

Objective: To observe the effect of beta-asarone on adriamycin-induced cardiomyocyte injury in suckling mouse. Method: Cardiomyocytes of neonate rat were cultivated for 72 hours and randomly divided into normal control group, adriamycin(ADR,2 mg·L^-1) model group,and beta-asarone(10,20,40 mg·L^-1) dose groups. MTT colorimetric method was deployed to detect cardiocyte survival rate,activities of medium lactate dehydrogenase(LDH), superoxide dismutase(SOD) and malondialdehyde(MDA) were detected,and protein expression of B cell lymphoma/lewkmia-2(Bcl-2) and Bcl-2 associated X protein(Bax) were detected by immune histochemical method. Result: Compared with normal control group, model group ADR cardiomyocyte shrinkage deformation,irregular,beat rhythm irregularities,frequency obviously slow down, numbers of survival cells were decreased, the leakage of LDH was increased; SOD activity was decreased; the contents of MDA were increased; protein expressions of Bcl-2 were reduce, protein expressions of Bax were increased (P <0.01 or P <0.05). And compared with ADR model groupand ADR model group, each dose group of beta-asarone had no changes in cardiomyocytes cell pulsation frequency, present centrality synchronization pulsing; cardiomyocytes survival rate was increased; the leakages of LDH was decreased;and the activity of SOD was increased; contents of LDH and MDA were decreased; protein expression of Bcl-2 was increased, protein expression of Bax were reduced (P<0.01 or P <0.05). Conclusion: Beta-asarone protects mice from adriamycin-induced cardio-toxicity by reduction of oxygen free radicals and apoptosis in mouse cardiac tissues.