Effect of Xinfukang Oral Liquid on CK-MM mRNA and CK-MM Protein Expression Level in Rat’s Heart Failure Model after Myocardial Infarction

Abstract:

Objective: To study the effect of Xinfukang(XFK)oral liquid on MM isoenzyme of creatine kinase(CK-MM) mRNA and CK-MM protein expression level in rat of heart failure model after myocardial infarction. Method: The rat heart failure mode after myocardial infarction was created by ligating the left anterior descending coronary arteries. All the rats were randomly divided into 4 groups:sham group, model group, XFK 5.4 g·kg^-1 group and captopril 0.1 g·kg^-1 group. After 24 hours of modeling, rats were ig given XFK and captopril for 4 weeks, Cardiac function, myocardial ultrastructure and key enzymes of myocardial energy shuttle, CK-MM mRNA and protein expression level were separately measured in all groups. Result: ①Compared with model group, in captopril group and XFK group CK-MM mRNA and protein expression increased (P<0.01). ②Compared with captopril group, in XFK group CK-MM mRNA expression level decreased (P<0.05). In XFK group CK-MM protein expression has no significant change. Conclusion: XFK oral liquid can effectively increase the expression level of CK-MM mRNA, as well as the protein content of CK-MM to improve myocardial energy shuttle and remodel the process of myocardial energy metabolism function.