Effect of Platelet Rich Plasma Combined with Bone Marrow Mesenchymal Stem Cells in Treatment of Rabbit Femoral Head Necrosis

Abstract:

Objective: To study effect of platelet rich plasma(PRP) combined with bone marrow mesenchymal stem cells(BMSCs) in treatment of rabbit femoral head necrosis. Method: Fifty rabbits were randomly divided into normal group, model group, PRP group (3%), BMSCs group (1×10⁶/mL) and combination group. The model of rabbit femoral head necrosis was made by operation. After 4 weeks of operation, the PRP group was implanted the PRP, the BMSCs group was implanted the BMSCs, the combination group implanted the PRP and BMSCs. Normal group was not treated. The rabbit femoral head necrosis were observed by HE trichrome coloration after 12 weeks of operation. The change of the trabeculae of bone, bone cells in the bone,fat cells were observed by light microscopy. Then PDGF protein in the rabbit femoral head was tested by Western blot. Result: Compared with the normal group, the trabecular of bone became thinner, decreased in bone cells,fat cells increased, empty osteocyte lacuna in the trabeculae were increased in model group (P<0.05). Compared with the model group, in all treatment groups trabecular bone became thicker, bone cells increased, fat cells decrease, empty osteocyte lacuna in the trabeculae were decreased and improved the expression of PDGF in the rabbit femoral head (P<0.05). Conclusion: PRP combined with BMSCs can effectively induce bone regeneration, improved the expression of PDGF and improve bone tissue recovery.