Enhancing Effect of Constituents Absorbed into Blood after Oral Administration of Liuwei Dihuang Wan on Killing Hepatocarcinoma Cells with HSV-tk/GCV System

Abstract:

Objective: To explore the enhancing effect on killing hepatocarcinoma cells of CBRH7919 by herpes simplex virus thymidine kinase and ganciclovir (HSV-tk/GCV) system together with constituents absorbed into blood after oral administration of Liuwei Dihuang Wan. Method: MTT assay was used to detect growth inhibition of CBRH7919 after being treated with tk/GCV system and constituents including loganin,morroniside,5-(methoxymethyl)-2-furoic acid,sweroside,paeonol and mixed drugs of these five compounds; also to detect growth inhibition of CBRH7919 after being treated with tk/GCV system and lymphocytic supernatant fluid activated by constituents. Result: There was no synergy killing effect on CBRH7919 between tk/GCV system and constituents; while there was synergy killing effect on CBRH7919 treated with tk/GCV system lymphocytic supernatant fluid activated by constituents together, witch showed significantly dose-effect relationship. Conclusion: The active substrate activated by constituents of Liuwei Dihuang Wan has synergy effect on HSV-tk/GCV system,and the endogenous active substrate may be the material basis of synergy effect of Liuwei Dihuang Wan on HSV-tk/GCV system.