Influence of Dihuang Yinzi on the Content of Serotonin Receptors in Hippocampal of Rat with Poststroke Depression

Abstract:

Objective: To observe influence of of Dihuang Yizi (DHYZ) on serotonin 1 A receptor (5-HT_1AR) and serotonin 2 A receptor (5-HT_2AR) mRNA express in poststroke depression rats, and further discuss mechanism. Method: The sixty healthy adult SD rats were randomly divided into 6 groups including blank control group, model group, the optimal solutions group, DHYZ high dose group, medium group, low group. Afterpoststroke depression model was established the corresponding drugs were given (hydrochloric acid prozac,DHYZ 450,150,75 mg·kg^-1. The real-time fluorescent quantitative RT-PCR method was used to assay 5-HT_1AR and 5-HT_2AR mRNA expression. Result: Compared with model group; DHYZ medium (0.76± 0.13), (1.21±0.21) and high dose groups (0.75±0.1), (11.25±0.26) increased the 5-HT_1AR mRNA expression, and decreased the 5-HT_2AR mRNA expression (P<0.05). Conclusion: DHYZ may raise 5-HT_1AR mRNA, and decrease 5-HT_2AR mRNA, which can explain mechanism of DHYZ in treatment of poststroke depression.