Abstract:Objective: To study the pharmacodynamics and ciliotoxicity of Zhibing nasal cavity in-situ gels (ZBISG). Method: Fifty mice were randomly divided into five groups, normal saline (0.8 g·kg-1) were given for blank group,aspirin (0.04 g·kg-1) was given orally for the pasitive control group and 0.4,0.8,1.2 g·kg-1 ZBISG given orally for ZBISG groups. Five minutes after administration, 0.6% acetate solution (0.1 mL·10 g-1) was injected intraperitoneally in control group and ZBISG groups. Then the time of writhing response appeared and the number of mice writhing response were observed and recorded. Analgesic rate was also calculated. The pain threshold increase rate was measured at before and 15,30,60,90 min after ZBISG administration using hot plate test. The nasal ciliotoxicity of ZBISG was evaluated with in vivo toad palate, normal saline of 0.5 mL was used as the control. Result: For the mice torsion reaction, there was significant differences between treatment group and blank group(P<0.01). Between ZBISG treatment group and aspirin group, the high dose group and medium dose group were significantly different compared with that of aspirin group(P<0.01), however low dose group did not show difference to aspirin group. And as the dosage increased, the effect of analgesic action increased. In the time when the writhing response appeared, there was significant differences between administration group and blank group(P<0.01). And as the dosage increased, the effect of analgesic action increased. There was obvious difference in the pain threshold increase rate at each time point between ZBISG group and blank group(P<0.01) or between ZBISG treatment group and aspirin group(P<0.01) or between the high, medium dose group and low dose group(P<0.01).ZBISG had no obvious effect on the ciliary movement. Conclusion: ZBISG is a kind of effective and safe preparation.
