Abstract:Objective: To broaden the application field of bamboo leaf extract and enrich the type of anti-fatigue supplements for sports training, the effect of bamboo leaf extract on free radical metabolism of rats experiencing high-intensity endurance training were studied. Method: Twenty-four SD rats were randomly divided into quiet control group, sport control group and dosed sport group, with 8 rats in each. Sport control group experienced 6 week treadmill training. Dosed sport group experienced 6 week treadmill training and were fed 300 mg·kg-1·d-1 bamboo leaf extract suspension. Quiet control group and sport control group were fed with the same volume of distilled water. After six weeks, the content or activity of Superoxide Dismutase(SOD), Total Antioxidant Capacity(T-AOC), catalase(CAT), Malondialdehyde(MDA), Reduced Glutathione(GSH), Glutathione Peroxidase(GSH-Px) in heart, liver and kidney of all groups and sport ability of sport control group and dosed sport group were measured. Result: The content or activity of SOD, T-AOC, CAT, GSH and GSH-Px in heart, liver and kidney significantly decrease and MDA content significantly increase, but taking bamboo leaf extract can inhibit the downward trend of antioxidant enzyme activities, reduce the content of MDA and increase exhaustive exercise time by 28.87%. Conclusion: Taking bamboo leaf extract can promote the metabolism of free radicals in rats experiencing high intensity exercise and improve their athletic ability. So it is suitable as raw material of anti-exercise fatigue supplements.