Studying of Apoptosis of SGC-7901 Cell Induced by Zhishi Xiaopi Wan

Abstract:

Objective: Study on the mechanism of Zhishi Xiaopi Wan treatment on Gastric cancer from cell proliferation, apoptosis and cycle. Method: Complete medium was mixed in negative control group;1×10⁴ cells/wells were seeded at 96 wells plate and divided into negative control, Zhishi Xiaopi Wan in 0.05,0.125,0.5,2,4 g·L^-1 concentration groups;the cells were treated with drugs for 24,48,72 h, and cell proliferation was detected by MTT method; 1×10⁵ cells/wells were seeded in 6 wells plate, and the drugs of low(0.125 g·L^-1),medium(0.5 g·L^-1),high(2 g·L^-1) concentration respectively were put into wells for 48,72 h, then the cell cycle and apoptosis of gastric cancer SGC-7901 cells were detected by flow cytometry(FCM). Result: Gastric cancer SGC-7901 cells were treated by the 0.125,0.5,2 g·L^-1 of Zhishi Xiaopi Wan, and its inhibition rate is in dose and time dependent. Zhishi Xiaopi Wan could increase SGC-7901 cells in G₀-G₁ phase percentage and promote apoptosis; The high,medium concentration was stronger than the lows. Conclusion: Zhishi Xiaopi Wan can inhibit the proliferation of SGC-7901 cell and there is in dose and time dependent, which mechanism may be related to drug block of cells to G₀/G₁ phase and induce cell apoptosis.