Establishment of Rat Spleen Yang Deficiency Syndrome Insomnia Model and Effect of Fuzi Lizhong Decoction on it

Abstract:

Objective: To explore effect of Fuzi Lizhong decoction on the expression of hypothalamic orexin mRNA and hypcthalamopitutary-adrenol(HPA) axis in insomnia rats with spleen Yang deficiency syndrome and therapeutic mechanism. Method: Handred rats were randomly divided into control group, model group,low-dose group, middle-dose group, and high-dose group 5 groups. each group included 20 rats. Model rats were ip p-chlorophenylalanine(PCPA) according to 400 mg·kg^-1, once a day for two days to establish insomnia model. low-dose group, middle-dose group, and high-dose group were ig given Fuzi Lizhong decoction respectively with 10,20,40 g·kg^-1 based on model group for 7 days. Result: Compared with control group, expression of orexin mRNA of model group increased significantly (P<0.01).Compared with control group, content of 5-hydroxy tryptamine(5-HT) of model group reduced significantly (P<0.01).Compared with control group, content of corticotropin-releasing bormone(CRH) of model group increased (P<0.05). Compared with control group, content of adrenocorticotropic hormone(ACTH) of model group increased significantly(P<0.01). Compared with model group, expression of orexin mRNA of treatment group reduced significantly(P<0.01). Compared with model group,content of 5-HT of treatment group increased significantly (P<0.01)and content of 5-HT in middle-dose group increased (P<0.05). Compared with model group,content of CRH of treatment group reduced (P<0.05). Compared with model group,content of ACTH of treatment group reduced significantly (P<0.01). Conclusion: Fuzi Lizhong decoction can reduce expression of Orexin mRNA and so that inhibit HPA axis to reduce stress reaction, which perhaps is one of mechanisms for insomnia treatment.