Optimization of Extraction Technology of Active Components from Scirpus yagara

Abstract:

Objective: To optimize extracting technology of sparstolonin B and sanleng diphenyllactone from Scirpus yagara. Method: Contents of sparstolonin B and sanleng diphenyllactone were determined by HPLC,chromatographic conditions were as follows:Hanbang column(4.6 mm×250 mm,5 μm),mobile phase of methanol-1% acetic acid(65:35),flow rate 1.0 mL·min^-1,column temperature 30℃,detection wavelength 385 nm,injection volume 10 μL.With extracting amounts of sparstolonin B and sanleng diphenyllactone as indexes,orthogonal test was adopted to investigate effects of the concentration and dosage of enthanol,extraction time,extraction times on technology. Result: Order of effects of each factors on yield of sparstolonin B was extraction times>ethanol concentration>ethanol consumption>extraction time,but effects of each factor on yield of sanleng diphenyllactone was in order of ethanol concentration>extraction times>extraction time>ethanol consumption.Optimum extraction technology was as following:reflux extracted 2 times with 10 times the amount of 70% ethanol,1.5 h per time;Average extracting amounts of sparstolonin B and sanleng diphenyllactone were 13.95,5.95 μg·g^-1,respectively. Conclusion: Optimized extraction technology was stable and feasible with high extraction efficiency;This established determination method was sensitive,accurate and reproducible.