Optimization of Ultrasonic-Assisted Enzymatic Hydrolysis Technology for Baohuoside Ⅰ from Icariin

Abstract:

Objective: To optimize ultrasonic-assisted enzymatic hydrolysis technology of baohuosideⅠ. Method: The content of baohuosideⅠ was determined by HPLC,chromatographic conditions were as follows:Diamond C₁₈ column(4.60 mm×250 mm,5 μm),mobile phase of acetonitrile-water(25:75),flow rate 1.0 mL·min^-1,column temperature 30℃,detection wavelength 270 nm.Taking bioconversion rate of baohuosideⅠ as index,based on single factor tests,effects of ultrasonic power,pH,temperature,enzyme dosage and reaction time on preparation technology were investigated L₉(3⁴) orthogonal design. Result: Optimal enzymatic conditions were:ultrasonic power of 200 W,pH of 5.0,reaction temperature at 40℃,reaction time of 4 h,enzyme dosage 5%.Under these conditions,conversion rate of baohuosideⅠ was 97.4%. Conclusion: Ultrasonic-assisted enzymatic method could improve conversion rate of baohuosideⅠ with low reaction temperature,short reaction time,little enzyme and other advantages,optimized process was stable,simple and suitable for industrial production.