Optimization of Extraction Technology for Total Polyphenols from Cinnamomi Cortex and Investigation of its Hypoglycemic Effect

Abstract:

Objective: To optimize extraction technology of total polyphenols from Cinnamomi Cortex and observe its effect on streptozotocin (STZ)-induced diabetic mice. Method: With yield of total polyphenols as index,effects of the concentration of ethanol,solid-liquid ratio,extraction temperature and extraction time on extraction technology were investigated by orthogonal test.Diabetic mice model was established with fasting plasma glucose(FPG) as index,successful modeling mice were divided into five groups,such as the blank control group,model control group,positive control group(metformin hydrochloride),low-dose of total polyphenols from Cinnamomi Cortex group,high-dose group,effect of total polyphenols from Cinnamomi Cortex on blood glucose of streptozotocin (STZ)-induced diabetic mice was investigated. Result: Optimum extraction process was as following:extracted 60 min with 10 times the amount of 30% ethanol at 80℃;Average yield of total polyphenols from Cinnamomi Cortex was 3.9%,the mass fraction of it was 39.1%.Before administration,FPG of each group were (6.33±1.27),(18.06±3.12),(18.71±3.51),(18.06±3.27),(18.41±3.54) mmol·L^-1,respectively;While after gavage 3 w,FPH were (5.36±1.06),(31.59±2.94),(25.08±3.35),(15.33±4.02),(12.50±3.85) mmol·L^-1,respectively. Conclusion: Optimized technology was scientific and reasonable,it could be used as basis for trial production of total polyphenols from Cinnamomi Cortex.Total polyphenols from Cinnamomi Cortex had significantly decrease fasting blood glucose levels in diabetic mice.