Effect of Pretreatment Methods on Content of Cholic Acid of Sheep Bile

Abstract:

Objective: To investigate effect of pretreatment methods on the content of cholic acid from sheep bile. Method: Cholic acid was prepared by direct drying method,saponification method,vacuum freeze drying method,centrifugation method,respectively.The content of cholic acid was determined by UV,amd effect of pretreatment methods on the content of cholic acid from sheep bile was investigated. Result: Effect of different processing methods on the content of cholic acid was great,contents of cholic acid from saponification crude products,saponification refined products,freeze-dried products,centrifugation drying products1,centrifugation drying products2 and gallbladder direct drying products were 63.41%,79.55%,39.67%,30.62%,41.71%,36.26%,respectively. Conclusion: Comprehensive evaluation with yield of dry products and the content of cholic acid,direct centrifugation combining with adding 95% ethanol removal of protein composition and vacuum freeze drying method were better than other methods.