Quality Standards of Pingxiao Granules

Abstract:

Objective: To establish quality standardS of Pingxiao granules. Method: Ephedrae Herba,Paeoniae Radix Alba and Cnidii Fructus in Pingxiao granules were identified by TLC;The content of ephedrine hydrochlorid was determined by RP-HPLC,chromatographic conditions were as follows:Hedera ODS2 column(4.6 mm×200 mm,5 μm),mobile phase of acetonitrile-0.1% phosphoric acid(3:97),flow rate of 1.0 mL·min^-1,detection wavelength 207 nm,column temperature at 30℃. Result: Spots of Ephedrae Herba,Paeoniae Radix Alba and Cnidii Fructus in Pingxiao granules on TLC could be well separated.Ephedrine hydrochlorid showed a good linear relationship at the range of 0.069 6-0.417 6 μg (r=0.999 0),the average recovery was 99.35% with RSD 1.84%,the contents of ephedrine hydrochlorid in three batches of Pingxiao granules were 0.202 2,0.209,0.207 2 mg·g^-1,respectively;Temporarily intended the content of ephedrine hydrochlorid should more than 0.17 mg per 1 g of this product. Conclusion: This established method was simple,specific,sensitive and reproducible,it was suitable for quality control of Pingxiao granules.