Simultaneous Determination of Four Iridoid Glycosides in Yinzhihuang Granules by HPLC

Abstract:

Objective: To develop an HPLC method for simultaneous determination of four iridoid glycosides (geniposide,shanzhiside,deacetyl asperulosidic acid methyl ester and genipin-1-β-D-gentiobioside) in Yinzhihuang granules. Method: Chromatographic conditions were as follows:Kromasil C₁₈ column (4.6 mm×250 mm,5 μm),mobile phase of acetonitrile(A)-0.05% trifluoroacetic acid solution(B) with gradient elution program(0-15 min,5%-6%A;15-23 min,6%-10%A;23-35 min,10%-22%A),flow rate 1.0 mL·min^-1,detection wavelength 238 nm,column temperature at 30℃. Result: The linear ranges of geniposide,shanzhiside,deacetyl asperulosidic acid methyl ester and genipin-1-β-D-gentiobioside were 0.334 4-3.344 0,0.041 5-0.415 2,0.046 6-0.466 4,0.093 1-0.931 2 μg,respectively;Average recoveries of these four ingredients were 97.3%(RSD 1.6%),99.6%(RSD 2.7%),97.6%(RSD 2.3%) and 99.2%(RSD 2.2%),respectively. Conclusion: This method was convenient and accurate,it could be used to improve quality standard of Yinzhihuang granules.