Determination of Buddleoside in Buddlejae Flos Formula Granules by HPLC

Abstract:

Objective: To establish a method for determining buddleoside in Buddlejae Flos formula granules. Method: Buddleoside was separated on Inertsil ODS₃C₁₈ column(4.6 mm×250 mm,5 μm) with methanol-0.1% phosphoric acid(55:45) as mobile phase,flow rate was 1.0 mL·min^-1,column temperature was at 20℃,detection wavelength was 327 nm,injection volume was 10 μL. Result: Buddleoside had good linear relationship within the range of 0.104 8-1.048 μg with r=0.999 6,the average recovery was 102.2% with RSD 1.9%. Conclusion: This method was simple,accurate and reproducible,it could be used to determine the content of buddleoside in Buddlejae Flos formula granules.

Key words:Buddlejae Flos;formula granules;buddleoside;HPLC