Study and Evaluation of Quality Control of Echinacea Purpurea Oral Liquid

Abstract:

Objective: To establish the quality standard and evaluation of Echinacea Purpurea oral liquid. Method: TLC test was based on silica gel plate with the upper solution of ethyl acetate-acetic acid-water (6:0.5:1) as mobile phase. The analytical column was Agilent Eclipse XDB-C₁₈(4.6 mm×250 mm, 5 μm) with acetonitrile and 0.3% phosphoric acid as mobile phase in gradient mode;the flow rate was 1.2 mL·min^-1;the detecting wavelength was set at 330 nm;the column temperature was set at 35℃ and the injection volume was 10 μL. At the same time, pH and relative density were determined. Result: Characteristic of Echinacea Purpurea oral liquid was clear. The linear range of cichoric acid was 0.004-0.128 g·L^-1(r=0.999 9), and the average recovery was 101.13% with RSD 1.41%. Conclusion: The method to identify cichoric acid is simple and has a great repeatability. The method to detemine the content of cichoric acid in Echinacea Purpurea oral liquid is simple, accurate with a good reproducibility. So the TLC and HPLC methods can be used for the quality of the oral liquid.