Determination of Two Components Content in Caesalpinia decapetala by UPLC

Abstract:

Objective: To establish UPLC method for the determination of two components in Caesalpinia decapetala. Method: Separation was performed at 45℃, on a ACQUITY UPLC BEH C₁₈ column (2.1 mm×150 mm, 1.7 μm) with a gradient solvent system of acetonitrile-0.1% aqueous formic acid as the mobile phase. The flow rate was 0.3 mL·min^-1 and the detection wavelength was 266 nm. Result: The linear response ranges of(1'R, 3'S, 5'R, 8'S, 2E, 4E)-dihydrophaseic acid-3'-O-β-D-glucopyranoside and (±) Protosappanin B were 0.497-79.6 mg·L^-1 (r=0.999 8), 1.50-240 mg·L^-1 (r=0.999 9), respectively. The mean recoveries of the two components were 100.9%(RSD 1.9%), 99.89%(RSD 1.8%). Conclusion: The method is simple and accurate, and is suitable for evaluating the quality of Caesalpinia decapetala.