Determination of Triptoquinone B in Tripterygium Glycosides by LC-MS Analysis and HPLC

Abstract:

Objective: To develop an analytic method for determination of triptoquinone B in tripterygium glycosides. Method: The HPLC-ESI-MS and HPLC-DAD methods were established. Triptoquinone B in tripterygium glycosides was quickly identified by comparing the retention time, UV absorption spectrum and quasi-molecular ion peak [M+Na]⁺ with the reference compound. The content of triptoquinone B in tripterygium glycosides was determined by HPLC. Result: One level mass spectrometry scanning results showed that the chromatographic peak of retention time t_R=13.2 min was identified as triptoquinone B, and its quasi-molecular ion peak [M+Na]⁺ was 353.1.The calibration curves of triptoquinone B was linear between 0.115-1.15 μg (r=0.999 9). The average recovery and the relative standard deviation were 99.32% and 0.7% respectively. Conclusion: The method is simple, accurate and could be used for determination of triptoquinone B in tripterygium glycosides.