Simultaneous Determination of Chlorogenic Acid and Buddleoside in Chrysanthemi Indici Flos by UPLC

Abstract:

Objective: To establish a UPLC method for simultaneous determination of buddleoside and chlorogenic acid in Chrysanthemi Indici Flos from different areas. Method: UPLC was used. The chromatographic separation was performed on a C₁₈ (2.1 mm×50 mm, 1.8 μm) column with gradient elute. The flow rate was 0.2 mL·min^-1. The detection wavelength was set at 326 nm and column temperature was maintained at 25℃. Result: Good linear relationship between the peak area and content of buddleoside and chlorogenic acid was achieved when the amount was within 3.0-120.0, 0.48-19.20 mg·L^-1;the recoveries were 99.43% and 98.67%;RSDs were 1.54% and 2.13% respectively(n=6). Conclusion: The UPLC analysis is speed, reproducible, accurate, efficient and convenient, which can detect the content of buddleoside and chlorogenic acid in Chrysanthemi Indici Flos.