Abstract:Objective: To establish an HPLC method for determination and characteristic spectrum of flavonoids in Citrus Fructus and Citri Sarcodactylis Fructus. Method: Chromatographic analysis was carried on the coloumn of Waters SunFireTM C18(4.6 mm×150 mm, 5 μm), the mobile phase was acetonitrile-0.1% phosphoric acid at the flow rate of 1.0 mL·min-1;the detection wavelength was set at 284 nm and the coloumn temperature was 30℃. Result: The flavonoids compounds were base-isolated in HPLC characteristic spectrum. The linear range of hesperidin was 0.173 6-1.215 2 μg(r=0.999 5), the average recovery was 101.19%(n=6) in Citrus medica, and the average recovery was 99.11%(n=6) in Citri Sarcodactylis Fructus. The linear range of naringin was 0.190 4-1.142 4 μg(r=0.999 5), the average recovery was 102.98%(n=6). Conclusion: The method is rapid, accurate and reliable, and can be applied to the quality control of Citrus Fructus and Citri Sarcodactylis Fructus.