Study on HPLC Fingerprint Analysis of Roasted Malt Formula Granules

Abstract:

Objective: To establish a method of fingerprint analysis on Roasted Malt Formula granules by HPLC. Method: The fingerprints chromatographic were obtained by HPLC with Megres5-C₁₈ column (4.6 mm×250 mm,5 μm) by gradient elution;the solvent system composed of acetonitrile-0.1% phosphoric.The wavelength was set at 316 nm, the flow rate was 1.0 mL·min^-1 and the column temperature was maintained at 30℃. Result: The HPLC specific chromatogram of Roasted Malt Formula granules showed 9 common peaks. The methodological results were good. The similarity of ten batches of samples exceeded 0.88. Conclusion: The fingerprints chromatogram can realize the analysis of a formulas as a whole with supplies references for improving the quality control of Roasted Malt Formula granules.