Effect of SiniSan Jiawei on TGF-β1 and its Receptor Gene and Protein Expression in Liver Tissue of Rats with Hepatic Fibrosis

Abstract:

Objective: To discuss the curative effect and action mechanism of Sinisan Jiawei flavored in treatment of hepatic fibrosis. Method: Eighty Wister rats were randomly divided into eight groups:normal group;model group;Sinisan group(4.0 g·kg^-1);high dose group, mediate dose group and low dose group (14,7,3.5 g·kg^-1) and prevention group(7 g·kg^-1) of supplemental Sinisan;colchicine group(0.2 mg·kg^-1). Except the norrmal groups,all the other groups were given pig serum to induce liver fibrosis twice a week, 0.5 mL/per rat for 10 weeks. By RT-PCR and immunohistochemical S-P method were used to monitor liver tissue transforming growth factor β₁(TGF-β₁) and its receptor gene and protein expression. Result: After treatment of Sinisan Jiawei flavored the rats liver tissue TGF beta 1 and its receptor gene and protein expression significantly lower (P<0.01)compared with the model group compared.Compared with colchicine group, the TGF-β₁ and its receptor gene and protein expression lowered more significantly (P<0.01). Conclusion: The action mechanism of Sinisan Jiawei flavored in treatment of hepatic fibrosis may reduce liver tissue TGF-β₁ and TGFR expression.