Effect of Lycopene on Mitochondrial Damage of PC12 Cells Induced by Rotenone

Abstract:

Objective: To investigate the protective effects of lycopene against rotenone-induced mitochondrial damage in PC12 cells. Method: All the experiments were carried out with PC12 in vitro,which were divided into 5 group,control,model(0.5 μmol·L^-1),lycopene(3,10,30 μmol·L^-1) preincubation.Cell viability was determined by CCK-8 assay;inverted phase contrast microscope was used to observe the cell morphological was;the ultrastructural changes of neuronal mitochondria were viewed under transmission electron microscope.The flow cytometry was used to detect the mitochondrial membrane potential(MMP). Result: CCK-8 assay showed that rotenone had cytotoxicity in PC12 cells,compared with the cells in the control group,cell viability was declined to 70.34%±2.81%(P<0.05), the pre-treatment of lycopene(3,10,30 μmol·L^-1) could significantly increase the PCI2 cell viability, cell viability was increased to 83.09%±3.15%,87.24%±2.15%,89.17%±2.26%(P<0.05). Compared with the cells in the model group,the number and morphology of neuronal mitochondria changed distinctly in the lycopene pretreated cells. Compared with the cells in the model group,the pre-treatment of lycopene(3,10,30 μmol·L^-1)increased mean fluorescence intensity of mitochondrial membrane potential to 202.24±26.28,226.21±9.71,238.83±10.29 (P<0.05). Conclusion: Lycopene can exert protective effects against rotenone-induced mitochondrial damage in PC12 cells.