Effect of Jianpi Yiqi Huoxue Decoction on SHR Releasing Vasoactive Substances by Regulating ACE2-Ang-(l-7)-MAS-AKT Signal Pathways Regulate Releasing Vasoactive Substances

Abstract:

Objective: To study the effect of the Chinese medicinethat Jianpi Yiqi Huoxue decoction on angiotensin converting enzyme 2(ACE2)-angiotensin (1-7)[Ang(1-7)]-MAS-protein kinase B(AKT)signal pathways in spontaneously hypertensive rats(SHR). Method: Sixty 24-week-old SHRs were randomly divided into six groups:SHR group (distilled water), perindopril group (perindopril 0.4 mg·kg^-1·d^-1), perindopril plus herb group (perindopril 0.4 mg·kg^-1·d^-1+10 g·kg^-1·d^-1of herb), high, medium and low herb group (40,20,10 g·kg^-1·d^-1). And Wistar kyoto rats(WKY) group. The changes of systolic pressure were measured by BP-98A intelligent sphygmomanometer. After six weeks' treatment, the content of Ang(1-7), ACE2, MAS, AKT was determined. Result: The systolic pressure in the perindopril group and the perindopril plus herb group was prominently decreased, compared with SHR group (P<0.01). The content of Ang(1-7), ACE2, MAS, AKT in the SHR group was lower than those in the WKY group, the perindopril group, the perindopril plus herb group, and the high dose of herb groups(P<0.01). Conclusion: Jianpi Yiqi Huoxue decoction can promote endothelial vasomotor factors secretion through the ACE2-Ang (l-7)-MAS-AKT signaling pathway.